Arun Upadhyay

4 Questions 10 Answers 0 Followers

Questions related from Arun Upadhyay

Can anyone suggest a simple protocol for analysing protein aggregates under scanning electron microscope. I need details of sample preparation?

Can anyone suggest the details of sample preparation. I want to reduce the use of uranyl acetate due to certain limitations with my sample.

08 August 2015 6,430 4 View

Equally loaded DNA seems to be unequal in amount on gel doc while doing agarose gel electrophoresis. What is the reason for this?

While loading the equal amount of DNA in all wells for agarose gel electrophoresis, I am not getting the equal visualization of DNA in all lanes. Is it because EtBr binds with different affinities...

07 July 2014 5,844 3 View

Why are my cells are not adhering on culture plates and getting rounded, but neither dying nor growing?

I am trying to revive my cells and I don't know what they're being affected by. I have thoroughly cleaned every corner of the culture room and incubator; done all possible cleaning practices to...

03 March 2014 214 11 View

What could be the reason that the pH of animal cell culture media is changed to 8.5 by putting them simply in air for 25 minutes?

I am using DMEM media with NaHCO3 and 10% FBS added with CO2 conc. 5%..

03 March 2014 7,673 2 View