Can anyone suggest a protocol for the simultaneous isolation of nuclei, mitochondria, and lysosomes?

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Kay Samuel

Axis- Shield produce OPTI-Prep which can be for gradient separation. Their website has protocols for using it for isolation of cells, organelles etc. It would be a place to start looking to develop a protocol

kay

Maren Catherina Podszun

Thank you Kay for the suggestion ! I will definitely look into that.