Why not Kjeldahl nitrogen from a typical acid solution? Bradford is not well suited IMO. You'd need to do both probably to show Bradford method as accurate.

Hi Diako Khodaei , a simple and fast procedure for protein determination in pasta (based on AOAC 930.25) is the Kjeldahl method.

Please find the attachment below. It will guide you through the whole process. Good luck.

bradford for detection of protein and its consentration is very good.

I think you can solve some of the sample and analyse it

You can use machine learning algorithms

The BCA protein assay could be used for quantitation of total protein in a sample