Can anyone suggest a glioblastoma cell line other than U87-MG?

More Jorrit De Waele's questions See All

Protocol to dissociate spheroids into single cells for flow cytometry?

Dear all, I am working with spheroids of U251 and U87 glioblastoma cells. Now, I would like to dissociate the spheroids for single cell flow cytometry analysis. However, either the spheroids are...

02 March 2018 9,622 7 View

Which markers are best to differentiate between human monocytes and macrophages?

In order to differentiate between human monocytes and (monocyte-derived) macrophages, which markers can best be used in flow cytometry?

08 September 2014 5,270 27 View

Does anybody know a positive magnetic NK cell isolation kit?

I would like to isolate NK from PBMC by positive magnetic selection to obtain an untouched, NK-less PBMC fraction. The only commercial kits with positive NK selection I came across, used a...

05 June 2014 9,528 6 View

What tests should be performed to confirm cancer stem cell identity of tumor cells?

I received cancer stem cell lines, but also plan to isolate such cells from tumor samples. Which tests should I carry out to confirm that I will be actually working with CSC?

31 December 2013 1,672 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Jorrit De Waele

Thanks for the helpful suggestions Rob, I think I'll go with LN-229.

Robert Kiss

The Hs683 model is also a "nice" one (see the attaced articles).

To the best of my knowledge, this is the sole hifg-grade oligodendroglioma "easy" to manipulate as xenografts and that mimics relatively well the clinical situation.

Best regards

Robert