Jorrit De Waele

5 Questions 14 Answers 0 Followers

Questions related from Jorrit De Waele

Protocol to dissociate spheroids into single cells for flow cytometry?

Dear all, I am working with spheroids of U251 and U87 glioblastoma cells. Now, I would like to dissociate the spheroids for single cell flow cytometry analysis. However, either the spheroids are...

03 March 2018 9,586 7 View

Which markers are best to differentiate between human monocytes and macrophages?

In order to differentiate between human monocytes and (monocyte-derived) macrophages, which markers can best be used in flow cytometry?

09 September 2014 5,215 27 View

Does anybody know a positive magnetic NK cell isolation kit?

I would like to isolate NK from PBMC by positive magnetic selection to obtain an untouched, NK-less PBMC fraction. The only commercial kits with positive NK selection I came across, used a...

06 June 2014 9,502 6 View

Can anyone suggest a glioblastoma cell line other than U87-MG?

Currently I use U87-MG, but I would like to add another glioblastoma cell line. Does anybody have a suggestion which other GBM cell line to use?

02 February 2014 948 2 View

What tests should be performed to confirm cancer stem cell identity of tumor cells?

I received cancer stem cell lines, but also plan to isolate such cells from tumor samples. Which tests should I carry out to confirm that I will be actually working with CSC?

01 January 2014 1,641 1 View