Does anyone here have an e-copy of Handbook of Solvent Extraction by Lo, Baird and Hanson?

My qualifying exams are the next week and my university library is out of copies! I would greatly appreciate your help.

06 January 2021 9,929 1 View

How does Q5 GC enhancer works?

I'm doing a PCR with Q5 enzyme. I would like to know how Q5 GC enhancer works so that I can decide whether to add it or not.

11 February 2019 7,083 7 View

Whether "lattice parameter" and "lattice constant" are refering to same constants like a,b,c in crystals?

In crystal structures, the lattice constants are defined as "a", "b" and "c". In few literature(s), the "lattice parameter" is used. Are they same?. Is there any difference?.

27 January 2019 7,088 4 View

What is the minimum cDNA template amount to use for BioTaq Polymerase reaction?

I am doing a PCR with BioTaq polymerase enzyme from Bioline. I am not seeing any positive bands on my gel. Does anyone know the minimum amount of template to use for this enzyme reaction?

15 October 2018 1,316 3 View

How useful is RIN value when you send out total RNA for miRNA sequencing?

I am extracting RNA from mouse tissue and would like to send the samples for sequencing, particularly looking for miRNAs in the samples. How useful and resourceful is the RIN value when the...

05 June 2018 3,300 3 View

Are there any specific microglial and astrocyte markers for primary cultures from postnatal mouse pups (P4-P7)?

Hi all. I am culturing primary astrocyte and microglia cells from postnatal mouse pups (P4-P7) and we are interested in looking at the gene expression specific for these cells types at this age....

06 March 2018 3,867 6 View

How to make the simulation faster in simulink ?

Hi..I am working on battery aging control in EV using PSO technique ...Right now I am aiming to reduce the simulation time and make the make the code efficient ..Can some suggest me how can I...

02 November 2017 4,860 3 View

What buffer can replace the binding buffer in DNA purification kits?

I have a QIAquick spin column but do not have the PB and PE buffers provided with it. I do know however that the binding buffer needs to have a chaotropic salt. Is there any buffer/solution with...

04 June 2017 1,750 2 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

06 August 2015 2,210 5 View

How can I obtain the band gap of the material based on the tauc plot if the absorption coefficient is unknown?

I have recorded the UV-absorption spectrum of my sample (bismuth titanate). I wish to know how we can determine the exact bandgap based on the tauc plot, if the absorption coefficient is unknown?

18 January 2015 2,901 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View