Harini Anandhi Senthilkumar

2 Questions 8 Answers 0 Followers

Questions related from Harini Anandhi Senthilkumar

Can anyone share their experiences working with triple negative cell lines to screen for bioactivity?

Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...

10 February 2016 6,160 3 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

In this case, I started with 300,000 cells. I used 0.25% trypsin and 750ul media while extracting them. 1ml PBS and 0.1% FCS were used to wash the cells after centrifugation. This is followed by...

07 August 2015 2,310 5 View