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Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...
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In this case, I started with 300,000 cells. I used 0.25% trypsin and 750ul media while extracting them. 1ml PBS and 0.1% FCS were used to wash the cells after centrifugation. This is followed by...
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