9 Questions 1 Answers 0 Followers
Questions related from Jo Be
Hello all, I'm currently immersed in research involving the Rosa26-tdtomato (Ai9) mouse line. We performed scRNA seq and analysis focusing on tdtomato-expressing cells but faced a challenge with...
02 February 2024 6,360 0 View
Hi researchers, I have a snRNA Seq dataset, and I am analysing it using R Seurat package. My task is to find out if the expression of a certain genes in the cells leads to differences in the...
25 August 2023 4,801 0 View
Hello guys, I am performing in IHC and I'm perfusing my mice and fixating them to extract the brain. I'm new to this and I have performed some perfusions recently but I don't like the...
20 March 2023 1,517 4 View
I'm looking for a specific and good marker to stain the neurons. I want to specifically use IHC. I want to analyze the morphology of the whole neuron therefore it has to mark not only the cell...
09 March 2023 4,095 2 View
I want to specifically visualize Granule cells of the Hippocampus and I wonder if there is a reporter mouse line exists for that? Thanks in advance.
09 March 2023 329 1 View
I sometimes see in the protocols that perfusion should be conducted with Ice-cold PBS and fixation with Ice cold 4% PFA, in other papers they write that solutions should be in room temperature....
17 February 2023 8,125 1 View
I want to analyze the dendritic diameter of Granul neurons in the hippocampus (in DG specifically) I wonder if there's specific marker than stains the whole neuron with dendrites and processes?
14 February 2023 9,024 1 View
I do qPCR often using the SYBR green. Everytime, I have to prepare the mix which contains the Primers, Water and the SYBR green mix. I wonder if this mix is stable in -20? Can I prepare a stock of...
07 February 2023 765 0 View
I had someone ordering me the new primers for my PCR and I just realized that the reverse and forward primers have the same sequence. is that possible and does it work? or maybe a mistake happened...
26 January 2023 5,791 3 View
