Jo Be

6 Questions 1 Answers 0 Followers

Questions related from Jo Be

Why my perfusion doesn't go well?

Hello guys, I am performing in IHC and I'm perfusing my mice and fixating them to extract the brain. I'm new to this and I have performed some perfusions recently but I don't like the...

20 March 2023 1,511 4 View

IF staining for Neurons?

I'm looking for a specific and good marker to stain the neurons. I want to specifically use IHC. I want to analyze the morphology of the whole neuron therefore it has to mark not only the cell...

09 March 2023 4,090 2 View

Double transgenic reporter mouse for granular neurons?

I want to specifically visualize Granule cells of the Hippocampus and I wonder if there is a reporter mouse line exists for that? Thanks in advance.

09 March 2023 305 1 View

Granule neurons marker for dendritic analysis?

I want to analyze the dendritic diameter of Granul neurons in the hippocampus (in DG specifically) I wonder if there's specific marker than stains the whole neuron with dendrites and processes?

14 February 2023 8,985 1 View

Is qPCR mix stable in -20 degrees? can agarose gel be pre-prepared and melted before use?

I do qPCR often using the SYBR green. Everytime, I have to prepare the mix which contains the Primers, Water and the SYBR green mix. I wonder if this mix is stable in -20? Can I prepare a stock of...

07 February 2023 729 0 View

QPCR Reverse and Forward Primers with the same sequence?

I had someone ordering me the new primers for my PCR and I just realized that the reverse and forward primers have the same sequence. is that possible and does it work? or maybe a mistake happened...

26 January 2023 5,784 3 View