Can anyone send me a detailed procedure of miRNA cloning in a mammalian expression vector?
To validate a miRNA target mRNA can we do it in vitro by adding everything in a test tube? If not, what are the difficulties and how can we overcome this?
08 September 2014 981 4 View
I am using realtime PCR approach to measure mtDNA copy number with mitochondrial tRNA or rRNA and nuclear B2M primers. I isolated DNA from both the samples control and treated then I did real...
06 July 2014 7,645 8 View
When I perform an MTT assay after adding DMSO, only a brown colour is appearing. I am working with suspension cells and I want to know the cytotoxicity of a compound. After 24 hours of seeding...
11 December 2013 7,328 15 View
I want to check the miRNA levels and miRNA transcript is short in length. How to design primer for a specific mature miRNA?
11 December 2013 9,940 7 View
I want to stain the mitochondria in suspension cells by using mitotracker red (Invitrogen). Can anybody explain protocol to observe them in fluorescence microscope?
11 December 2013 829 12 View
Buffer composition is 50mM tris pH7.5(p.I 6) 150mM Nacl (tried upto 300mM) 5mM BME 1mM pmsf 10% glycerol. Even it is also aggregating after NI -NTA purification but I am diluting protein then it...
11 December 2013 9,452 6 View
If I want to design primers or mature form of a miRNA it is very short, so how to get primers. Can anybody explain in detail?
08 September 2013 937 2 View
When I am performing an experiment I could not observe the any signal. Usually I use FITC tagged antibody but I am suspecting that I have problem with sample preparation and cells may be damaging.
08 September 2013 8,117 14 View
I am working on the megakaryocyte development. I need this cell line for my study.
02 March 2013 5,341 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View