10 Questions 13 Answers 0 Followers
Questions related from Ram Babu Undi
To validate a miRNA target mRNA can we do it in vitro by adding everything in a test tube? If not, what are the difficulties and how can we overcome this?
09 September 2014 962 4 View
I am using realtime PCR approach to measure mtDNA copy number with mitochondrial tRNA or rRNA and nuclear B2M primers. I isolated DNA from both the samples control and treated then I did real...
07 July 2014 7,627 8 View
I want to check the miRNA levels and miRNA transcript is short in length. How to design primer for a specific mature miRNA?
12 December 2013 9,921 7 View
When I perform an MTT assay after adding DMSO, only a brown colour is appearing. I am working with suspension cells and I want to know the cytotoxicity of a compound. After 24 hours of seeding...
12 December 2013 7,294 15 View
Buffer composition is 50mM tris pH7.5(p.I 6) 150mM Nacl (tried upto 300mM) 5mM BME 1mM pmsf 10% glycerol. Even it is also aggregating after NI -NTA purification but I am diluting protein then it...
12 December 2013 9,430 6 View
I want to stain the mitochondria in suspension cells by using mitotracker red (Invitrogen). Can anybody explain protocol to observe them in fluorescence microscope?
12 December 2013 803 12 View
If I want to design primers or mature form of a miRNA it is very short, so how to get primers. Can anybody explain in detail?
09 September 2013 908 2 View
When I am performing an experiment I could not observe the any signal. Usually I use FITC tagged antibody but I am suspecting that I have problem with sample preparation and cells may be damaging.
09 September 2013 8,091 14 View
I am working on the megakaryocyte development. I need this cell line for my study.
03 March 2013 5,323 1 View
Can anyone send me a detailed procedure of miRNA cloning in a mammalian expression vector?
12 December 2012 635 0 View