What could be the cause of a severe drop in DO during diel oxygen measurements?

I am conducting a mesocosm experiment in 68 L rubbermaid totes examining the differences between gamma irradiated tailings waste (treatment) and non-irradiated tailings waste (control), we believe...

05 December 2016 8,460 2 View

Which user friendly software would you recommend for concatenation of DNA sequences?

I have eleven genes from the a segmented genome virus. I have multiple sequence alignment files and would like to concatenate the 11 genes together.

26 April 2016 5,441 2 View

Who can help me with collecting willow catkin flies in Belgium?

28 February 2016 3,861 1 View

Does anyone have information about social practices linked to irrigation canals?

14 January 2015 1,546 5 View

How do I get the age range (95% HPD) displayed at each node of an MCC tree in Figtree?

I have a maximum clade credibility tree drawn and I am trying to get the age range at each node but I don't seem to get it right. How do I get that? I have the node bars displayed but I need the...

17 December 2014 7,978 6 View

What is the simplest, user friendly but very reliable gene recombination detection software?

I need to determine whether any recombination events have occurred in genes I have sequenced.

25 June 2014 9,709 2 View

How can I get more than 100 hits when I do a nucleotide BLAST at the NCBI website?

I need to retrieve about 1000 hits from the GenBank to use for my analysis.

19 June 2014 1,762 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View