What could be the cause of a severe drop in DO during diel oxygen measurements?

I am conducting a mesocosm experiment in 68 L rubbermaid totes examining the differences between gamma irradiated tailings waste (treatment) and non-irradiated tailings waste (control), we believe...

05 December 2016 8,460 2 View

Which user friendly software would you recommend for concatenation of DNA sequences?

I have eleven genes from the a segmented genome virus. I have multiple sequence alignment files and would like to concatenate the 11 genes together.

26 April 2016 5,441 2 View

Who can help me with collecting willow catkin flies in Belgium?

28 February 2016 3,861 1 View

Does anyone have information about social practices linked to irrigation canals?

14 January 2015 1,546 5 View

How do I get the age range (95% HPD) displayed at each node of an MCC tree in Figtree?

I have a maximum clade credibility tree drawn and I am trying to get the age range at each node but I don't seem to get it right. How do I get that? I have the node bars displayed but I need the...

17 December 2014 7,978 6 View

What is the simplest, user friendly but very reliable gene recombination detection software?

I need to determine whether any recombination events have occurred in genes I have sequenced.

25 June 2014 9,709 2 View

How can I get more than 100 hits when I do a nucleotide BLAST at the NCBI website?

I need to retrieve about 1000 hits from the GenBank to use for my analysis.

19 June 2014 1,762 4 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View