I'm looking for ways of analysing discoure complition tasks and ways of devising them, any suggestions?

I am an MA student working on pragmatics, specifically DCTs (discourse completion tasks). do you happen to know any books or articles on how to write and analyze DCTs? or is there any source for...

28 July 2020 5,136 2 View

Do you know any solvents to remove insects from the sticky traps?

We have been using thinner to remove the insect specimens from sticky traps. However, we would like to use other solvents with no toxic effects.

15 March 2020 6,789 12 View

Does anybody have an Android ".csv" Malware Data-set?

17 December 2019 761 3 View

I run GSSHA model in WMS software but the result was not reasonable what is the problem?

i do it with 32 mm precipitation

05 August 2019 8,187 2 View

Hi, i want to work in targeted cpp-mediated delivery of pDNA. but i do not know if DNA can stably reach to nucleus?

i want to design a cell penetration peptide for delivery of pDNA. that this platform targeted with a tumor targeting peptid

24 April 2019 6,763 2 View

Why a plasmid in same condition give different amount of supercoil?

Did sequences affected amount of supercoil? Regardless of extraction in low temperature other factors can be handle for earn more supercoil? I have several plasmid that some of them ( such as...

11 January 2019 6,882 3 View

How we can calculate frequency of 52 bar truss?

I'm using the data available in the literature, I calculated the frequency but the same results can not be obtained. I used lumped mass matrix for calculating Mass matrix. M=ro*A*L/2*[1 0 0 0 0 0...

27 February 2018 7,155 5 View

What is the best software/method you would suggest to detect the differences between DNA sequences and interpret them?

For months I tried to detect the bacteria DNA in Fish tissues which were artificially infected. Recently I saw sharp bands repetitively present in some of the infected fish tissues, but these...

22 December 2017 8,324 5 View

What has been the "highest Saffron yield" you have recorded in the first year of planting in your research?

I do know about highest recorded Saffron yield in different studies, but your private experience with some explanations is intended here.

04 November 2017 8,482 2 View

Correlation between drugs in rural colleges and urban colleges?

Doing research between drug use in rural colleges and urban colleges would like to see if any relationships have been found

01 November 2017 6,909 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View