Is there any analysis of genetic risk for IBD stratified according to microbiota composition?

Is there any study out there where the genetic risk for IBD has been addressed in the context of a certain microbiota composition, say enterotype, or according to presence/absence of indicator...

10 November 2017 5,607 3 View

Can I selectively remove host cells from mouse blood while retaining intact bacteria?

To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...

09 October 2017 6,326 3 View

Preservation of bacterial transcriptome upon cooling?

Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...

08 September 2017 7,069 4 View

How to collect microbiota samples from neonatal mice for 16S sequencing?

How can I best collect samples from the small intestinal (ileal) microbiota of neonatal mice (e.g. postnatal day 3 or 7) for 16S amplicon sequencing? The neonatal intestine is very thin and...

05 June 2017 2,362 3 View

Metabolomic analysis of formaldehyde-fixed and/or permeabilized bacteria possible?

08 September 2016 4,785 2 View

Can anybody recommend an antibody and protocol to stain murine TLR2 in immunofluorescence histology?

We are struggling with the staining of murine TLR2 in PFA-fixed mouse cryosections. There are antibodies that work well for flow but up to now they didn't stain anything at all in PFA-fixed...

04 May 2014 236 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View