7 Questions 31 Answers 0 Followers
Questions related from Jakob Zimmermann
I want to isolate plasmid DNA from feces of mice mono-colonised with a plasmid-carrying E. coli and I'd like to avoid contamination with genomic DNA as much as possible. Any hints?
05 May 2018 5,218 1 View
Is there any study out there where the genetic risk for IBD has been addressed in the context of a certain microbiota composition, say enterotype, or according to presence/absence of indicator...
11 November 2017 5,687 3 View
To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...
10 October 2017 6,376 3 View
Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...
09 September 2017 7,128 4 View
How can I best collect samples from the small intestinal (ileal) microbiota of neonatal mice (e.g. postnatal day 3 or 7) for 16S amplicon sequencing? The neonatal intestine is very thin and...
06 June 2017 2,425 3 View
Has anybody compared the metabolomic (mass spectrometric) profile of native versus formaldehyde-fixed bacteria? Is it comparable? Or even of bacteria that have been permeabilized with a...
09 September 2016 4,852 2 View
We are struggling with the staining of murine TLR2 in PFA-fixed mouse cryosections. There are antibodies that work well for flow but up to now they didn't stain anything at all in PFA-fixed...
05 May 2014 298 1 View
