Can anyone recommend a good DNA extraction protocol for flagellated protozoa?

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Vincent Lefebvre

Dear Sheryl,

I would recommend a chelex DNA extraction for your cultures, it's quick and cheap so it's worth trying.

Chaoqun Yao

I use QIAGEN kits. They work well such as Leishmania protozoa.

Muhammad Awais Zahid

QIAGEN kits would work more efficiently

I use Qiagen myself but I thought MoBio was approximately the same so I recommended to try something else :)

Sheryl Murdock

I have used many of these kits before and had great luck with bacteria and archaea. The MoBio kit at least is not working on my flagellates.