Can anyone recommend a good DNA extraction protocol for flagellated protozoa?

More Sheryl Murdock's questions See All

What are the impacts of regional variations in antibiotic resistance patterns on patient outcomes, and what can be done to address these variations?

This question was asked to understand how variations in antibiotic resistance across different regions affect the way patients are treated and how their health outcomes are affected. The inquiry...

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Amino acids - how to convert from g/kg of sample weight to mg/g of protein?

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What are the key characteristics of intentional communities (gated communities, farmsteads, cluster sites) for people with IDD?

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Is there any needs assessment tool for children/adults wtih disability?

I want to find some tools to assess the comprehensive rehabilitation needs of children/adults wtih disability, including educational needs, vocational needs, medical needs and social needs.

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Love the idea of this. Will the project be global or country-centric?

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What is the possibility of DLS Standard Results being masked by diluent?

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Can anyone recommend a questionnaire that can be used to assess if an individual is a victim of bullying?

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Is there a sufficient amount of participants needed to calculate a Cronbach's Alpha and Multiple Regression?

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Is there a problem with my RNA pellet?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Low-yield gel extraction problem?

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The question is how to use Wavenet transform?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Vincent Lefebvre

Dear Sheryl,

I would recommend a chelex DNA extraction for your cultures, it's quick and cheap so it's worth trying.

Chaoqun Yao

I use QIAGEN kits. They work well such as Leishmania protozoa.

Muhammad Awais Zahid

QIAGEN kits would work more efficiently

I use Qiagen myself but I thought MoBio was approximately the same so I recommended to try something else :)

Sheryl Murdock

I have used many of these kits before and had great luck with bacteria and archaea. The MoBio kit at least is not working on my flagellates.