Immunostaining of common bacteria in FFPE samples?

Dear Collegues, If we want to perform immunohistochemistry /immunofluorence staining from FFPE human biopsy (vein) samples and study the presence of LPS and or common pathogen like Porphyromonas...

01 December 2020 5,744 2 View

Maiden vs married name?

When I claimed authorship of papers written under my maiden name, they have now appeared under my married name, and I am worried this will cause confusion. How do I fix this issue?

12 August 2020 6,936 5 View

Anyone with experience in control of a Shimadzu HPLC with MassLynx?

I want to control a Shimadzu LC-10 (Controller: SCL-10Avp, pumps: LC-10ADvp, autosampler: SIL-10ADvp) coupled to a Waters Quattro Premier XE with Masslynx 4.1. The LC is connected via a serial...

04 August 2019 7,345 4 View

What is a fair price for scientific literature?

Recently, there is an increasing discussion concerning the price for scientific literature. At university, it is no problem to get adequate information for free but in non-university villages, it...

24 April 2018 9,464 5 View

Meta-analysis of diagnostic test accuracy when studies report multiple thresholds?

14 July 2015 3,085 4 View

Can anyone help with creating a stable knockdown cancer cell line to be used for establishing tumour-bearing mouse model?

I would like to stably knockdown my GOI in the E0771 cell line (mouse breast cancer), which I will then inject into mice to establish a tumour-bearing model. However, I have never done this before...

25 May 2014 4,865 6 View

Can anyone recommend a ferrozine assay protocol (for tissue samples)?

Can anyone share a protocol for a ferrozine assay for tissue samples that is reproducible, including the lysis protocol? Also, what is the minimal amount of iron detectable using this protocol and...

25 March 2014 7,981 1 View

High-molecular weight reference protein?

Does anyone know of a high-molecular weight reference protein? Preferably, it has to be larger than 250kDa, but larger than 120kDa will also do. Also, it needs to be expressed in epithelial cell...

09 December 2013 4,093 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View