Can anyone recommend a good antibody for staining CD3+ cells for FACs analysis?

More Vasant Janakiraman's questions See All

Is it a good idea to start crowd funding for the research activities?

Since Research fundings are always limited in most countries, is it a good idea to set up crowdfunding? Thanks

31 March 2024 7,333 0 View

Water absorption and Oil absorption ASTM standard test methods for reactive alumina or fine alumina oxide powders. ?

I am interested to understand the ASTM/Standard test methods and instruments which are being used for the Water absorption and Oil absorption analysis of Reactive alumina or fine alumina oxide...

15 July 2023 631 1 View

If an author has more publications and less citations, what does it mean?

Please tell me how to calculate if an author is engaged in meaningful research and publications (quality or needed NOT quantity) ?

01 January 2023 7,838 4 View

How to normalise extra cellular secreted protein in western blotting?

If cellular lysates are used, GAPDH, Beta Actin would serve as house keeping genes for normalising the expression. Incase of secreted protein, what would be the best procedure to normalise the...

14 December 2020 8,822 0 View

Is it okay to use SYTOX stain along with PE antibodies for FACS analysis using Attune - FACS analyzer?

29 June 2020 5,267 0 View

Difference in results from two Abaqus/Explicit simulations?

Hi As part of a validation of a bigger model, I'm trying to calculate the work done in deforming two Truss Elements (T3D2). One end of these elements is ENCASTRED, while the other end is linked...

03 March 2020 8,805 4 View

Should I include studies in google scholar in my systematic review or only studies indexed in PubMed, Embase, Sci cit index and medline?

Dear all, I am conducting an Epidemiological systematic review and MA, during my search of database I found some 10 studies in PubMed, EMBASE, MEDLINE, Science Citation Index. Additionally, while...

06 January 2020 315 11 View

What is the prognosis of portal hypertension , Cirrhosis of Liver with Ascitis?

A case with portal hypertension and Cirrhosis since 4 years having Ascitis since 1 month. What will be the prognosis specific to this case and in general as well?

04 January 2020 1,124 17 View

What type of springs are best for maximum energy storage?

I would like to know what type of springs are best, practically for storing energy temporarily without taking up too much volume. Are constant force springs any better than torsion springs in this...

19 June 2019 5,614 0 View

Please help me with identifying papers published in predatory journals in health sciences?

During my recent project work in the field of physiotherapy, I had to download papers for review purpose. After reading them I realized they lack standards and quality to be published. And wasted...

25 October 2018 4,677 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Vasant Janakiraman

Thanks

Fatma Abdel Wahab

Please clarify your question

If you want to sort CD 3 Positive cells or just only to do staining

Fatma Abdel Wahab only for staining

@Vasant in our routine in Human Diagnosis , I had worked with FITC CD 3 from beckman coulter and ECD CD3 from Beckman coulter and both worked well