How to normalise extra cellular secreted protein in western blotting?

Narayanan Janakiraman @Narayanan-Janakiraman

15 December 2020 0 9K Report

If cellular lysates are used, GAPDH, Beta Actin would serve as house keeping genes for normalising the expression. Incase of secreted protein, what would be the best procedure to normalise the expression?

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