If cellular lysates are used, GAPDH, Beta Actin would serve as house keeping genes for normalising the expression. Incase of secreted protein, what would be the best procedure to normalise the expression?
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...
14 May 2024 7,717 2 View
Since Research fundings are always limited in most countries, is it a good idea to set up crowdfunding? Thanks
31 March 2024 7,333 0 View
I downloaded QCADesigner 2.0.3 tool. but i am not able to open simulation type set up. once i click on that option entire window suddenly goes on ..Please help me to solve the issue
06 December 2023 853 1 View
I saved the waveform from QCADesigner tool with an extension of .EPS. But when I open the file in EPS viewer it is not opened and EPS window suddenly closed. Is the way correct to open a file in eps
06 December 2023 559 2 View
Answer
14 September 2023 2,271 1 View
INTERNAL CORPORATE SOCIAL RESPONSIBILITY MODEL TO INCREASE ORGANIZATIONAL PERFORMANCE IN MALAYSIAN PRIVATE HIGHER EDUCATION INSTITUTIONS IN KLANG VALLEY, MALAYSIA Based on the above topic, what...
13 July 2023 7,692 2 View
I'm not able to reach Professor Reynolds directly and none of the authors of the papers that have used his scale have replied to my emails. If anyone has used this scale then please let me know if...
12 July 2023 7,621 2 View
Hi everyone. I have LC-MS/MS data and the trial version of the Bruker compass data analysis software. I need to identify certain compounds in the chromatogram. Doing so manually is proving to be...
30 May 2023 8,087 0 View
Ethics approval for questionnaire validation study
11 May 2023 8,439 5 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!
27 July 2024 6,271 5 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
Is any validated questionnaire available to measure "House money effect" bias in the financial market (Likert scale)?
11 July 2024 6,772 4 View
As a marketing student I am performing research on client's attitude or barriers when they choose or hire architect or interior designer to construct their houses or renovate their home
08 July 2024 1,263 0 View