Is it okay to use SYTOX stain along with PE antibodies for FACS analysis using Attune - FACS analyzer?
Since Research fundings are always limited in most countries, is it a good idea to set up crowdfunding? Thanks
31 March 2024 7,333 0 View
I am interested to understand the ASTM/Standard test methods and instruments which are being used for the Water absorption and Oil absorption analysis of Reactive alumina or fine alumina oxide...
15 July 2023 631 1 View
Please tell me how to calculate if an author is engaged in meaningful research and publications (quality or needed NOT quantity) ?
01 January 2023 7,838 4 View
If cellular lysates are used, GAPDH, Beta Actin would serve as house keeping genes for normalising the expression. Incase of secreted protein, what would be the best procedure to normalise the...
14 December 2020 8,822 0 View
Can anyone recommend a good antibody for staining CD3+ cells for FACs analysis?
16 May 2020 446 6 View
Hi As part of a validation of a bigger model, I'm trying to calculate the work done in deforming two Truss Elements (T3D2). One end of these elements is ENCASTRED, while the other end is linked...
03 March 2020 8,805 4 View
Dear all, I am conducting an Epidemiological systematic review and MA, during my search of database I found some 10 studies in PubMed, EMBASE, MEDLINE, Science Citation Index. Additionally, while...
06 January 2020 315 11 View
A case with portal hypertension and Cirrhosis since 4 years having Ascitis since 1 month. What will be the prognosis specific to this case and in general as well?
04 January 2020 1,124 17 View
I would like to know what type of springs are best, practically for storing energy temporarily without taking up too much volume. Are constant force springs any better than torsion springs in this...
19 June 2019 5,614 0 View
During my recent project work in the field of physiotherapy, I had to download papers for review purpose. After reading them I realized they lack standards and quality to be published. And wasted...
25 October 2018 4,677 4 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View