Hi,
I would appreciate if any one can help me to find a cheap and reliable technique to measure gas pressure and amount in a serum bottle?
try using an old fashioned glass syringe. The plunger is typically low friction and so will pop up when the needle enters a culture flask under pressure.
try using an old fashioned glass syringe. The plunger is typically low friction and so will pop up when the needle enters a culture flask under pressure.
another old fashioned method is to use small tube to get the gas into a reversed graduated cylinder full of water.
The glass syringe is definitively very good. It is easy to calibrate, by adding several known gas volumes, in a known headspace. The R2 is also very nice. Despite that, it is a conservative method, as you can return the gas if you need. If you do not need a conservative method, you can use two small tubes connected to a vessel full of an acid solution (if you want to measure biogas), or a basic solution (if you want to measure methane). One of the tubes is connected to your bottle, and the other release the liquid to graduate cylinder. Please see the figure. I suggest you keep the tubes closed until pressure rise, in order to avoid liquid returning to your bottles. Good Luck!
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