How to compute the distance in km between two points inside the Earth?

Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...

02 February 2021 3,455 4 View

How can I quantify background noise in a bioacoustic file?

I am using Audacity to analyse the presence of birds and frogs recorded with a SM4 recorder (Wildlife Acoustic). Background noise (rainfall, wind) is an important variable to consider that can...

28 January 2021 2,989 3 View

How can I project 3D objects on 2D images??

Hello! From 3D images stacks I have recovered some objects. Now I need to project those objects on 2D for further analysis. Any idea, any software, plugin, etc?

21 January 2020 4,144 4 View

Does anybody knows if there is a metodology of acute ethanol toxicity for neuroprotection studies that uses functional foods?

I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...

03 January 2020 9,709 2 View

Is there a way to normalize my western blot bands since i loaded equal volume of seminal plasma (not based on protein concentration)?

I loaded 10ul of raw seminal plasma sample + 10 ul Bolt LDS buffer +4ul Bolt reducing reagent. I did the transfer using iBlot2 dry transfer system and used One Pierce ECL protein detection kit....

06 May 2019 6,912 3 View

Is there an acceptance criteria or limit for swelling index/behavior for sustained release matrix tablets?

I am doing a project on Matrix Tablets using natural products as binders (release retardant polymer) and I came across Swelling Behavior/Index. Is this necessary? I know swelling is vital for gel...

03 December 2017 8,272 2 View

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy?

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy? Solution A is pink while Solution B is green in color. If I will mix the...

16 October 2017 6,761 3 View

In identifying microorganisms using BLAST search, what is the purpose of converting the reverse sequences to reverse complement?

I have a 27F and 1492 R primers. And I wonder why the R primers should be converted to its reverse complement.

03 August 2017 6,530 2 View

Why I have low reproducibility in my real time pcr?

Hello , I'm quantifying bacteria by real- time PCR . I use plasmids with the target sequence as standards. The reproducibility is not good, one time i have 95% of efficiency and the next day is...

18 July 2016 4,605 7 View

How I can know the degree of crosslinking of a polymer?

Is it known that there is a variation in the degree of crosslinking varying synthesis parameters, but that technique I can use to know this degree?

28 April 2015 3,119 6 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View