Using two-tailed test for directional hypothesis?

In my study, I have used Process Macro to test the proposed relationships. The hypothesis is directional and two-tailed test has been deployed. Can anyone please clarify how to justify using...

06 February 2021 3,023 3 View

Why a colorimetric assay is usually not in green?

Various colorimetric assays we use are blue/purple/pink/red but not in green shades. Is there any particular reason for this?

30 May 2020 5,834 1 View

Is there a fluorescent dye or any method with which Chitosan can generate fluorescence?

I have generated polyelectrolyte complex in the form of fibers using Chitosan and polygalaturonic acid and in order to observe the fibers under confocal microscope they have to generate fluorescence.

11 March 2020 6,627 4 View

What are some physical methods to determine binding affinity of a protein?

What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...

09 March 2020 8,053 8 View

Is there a way to convert a 2D matrix into 1D vector which preserves the joint distribution among the columns in the matrix?

I have a matrix where each column represents a feature and each row represent a data point. I am trying to convert this matrix into a 1D vector which can contain the joint distribution among the...

01 March 2020 6,408 3 View

How do I interpret the below test for Uni-variate normality?

Hi, I have a large data sample with 15k rows and I am trying to perform uni-variate normality analysis on the dataset. I have come to understand that normality tests are really sensitive towards...

11 March 2019 3,041 4 View

How to acidify samples when using GC-FID for free Volatile Fatty Acid quantification?

Hi everyone, I use the GC-FID with the StabilWax DA column (http://www.restek.com/chromatogram/view/GC_CH00284) for volatile fatty acid (VFA) quantification (C2 - C7 acids). For this, I use 2%...

15 March 2018 9,752 15 View

What kind of ANOVA to use for my analysis with four factors?

I am doing a study based on the speech measures with gender, health condition, different passages read and different number of sentences from each passage. The number of levels in these factors...

16 January 2018 310 6 View

Curious if desalination plants publish their utilization rates? i.e. how much of their built capacity they produce on a day-to-day basis?

In other words, I am looking for daily/weekly/monthly water production data of all large-scale desalination plants (above 20,000 m3/day).

11 October 2017 6,261 3 View

High GAPDH values in qPCR

I use GAPDH as reference gene in qPCR runs. For one of my samples, I am repeatedly getting high Ct values of GAPDH (27-33). it's 20 or less for all other samples and control. I tried using its...

06 July 2017 7,543 5 View

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02 March 2021 3,060 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View