How can I estimate Kd value of protein-protein interactions, If I have measurements only in linear range?

How can I estimate Kd value or any parameter showing the affinity of protein-protein interactions, If I have measurements(band intensities from SDS PAGE) only in linear range? Any methods, other...

08 September 2017 9,620 4 View

Can anyone please suggest me the best working concentration of Hoechst stain for neuroblastoma cell lines?

Can anyone please suggest me the best working concentration of Hoechst stain for neuroblastoma cell lines??

02 March 2016 5,964 1 View

What are the available tools & techniques to figure out a gene coding for a distinguishable phenotype in lizards ?

What are the available tools & techniques to figure out a gene coding for a distinguishable phenotype in lizards ?

11 December 2015 9,414 0 View

If two proteins run in sds page with different molecular weights, if both show bands with same intensity, does it mean they have same concentration?

If two proteins run in sds page with different molecular weights(having difference of 60 KDa),if both shows bands with same intensity,does it mean they have same concentration?

10 November 2015 6,879 3 View

Can anyone please suggest the name of any software or website useful for generating animated cartoon videos for explaining biological reactions ??

Please suggest the name of any user friendly free software or websites used for generating cartoon videos or animated pictures for explaining molecular reactions involving biological molecules and...

08 September 2015 7,439 0 View

After pelleting down the cells for plasmid isolation or protein purification , why did I find black substances along with the white pellets?

06 July 2015 6,815 5 View

Amount of a polyhistagged protein in an elutionbuffer having 30mM imidazole after an elution with a 350mM imidazole containing elutionbuffer is more?

Amount of a polyhistidine tagged protein in an elution buffer having 30mM imidazole just after an elution with a 350 mM imidazole containing elution buffer is significantly more.Can anyone please...

02 March 2015 6,800 9 View

What is the average distance in base pairs between successive restrictive digestion cut sites?

The recognition sequence for the restriction endonuclease AstWI is GRCGYC where R is any Purine and Y is any Pyrimidine base. In a truly random DNA sequence what will be the average distance...

11 December 2014 5,619 1 View

In SDS PAGE, actin crosslinking doesn't show any significant increase in mass comparing actin bindings - any thoughts?

Can anyone help me to explain while running my protein samples in SDS PAGE, Actin crosslinking doesn't make any significant increase in molecular mass comparing actin binding protein samples.

07 August 2014 6,241 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

How to compare different models with different inputs and data length?

I want to run 5 different models to estimate stream flow. In order to optimize the characteristics of these models I use Taguchi method. So I have to run different models according to the Taguchi...

11 February 2021 7,986 6 View