How can I estimate Kd value of protein-protein interactions, If I have measurements only in linear range?

How can I estimate Kd value or any parameter showing the affinity of protein-protein interactions, If I have measurements(band intensities from SDS PAGE) only in linear range? Any methods, other...

08 September 2017 9,620 4 View

Can anyone please suggest me a technique for collecting insect secretions in nano litre range?

Can anyone please suggest me a detailed technique for collecting insect secretions in nano litre range?

02 March 2016 8,953 4 View

Can anyone please suggest me the best working concentration of Hoechst stain for neuroblastoma cell lines?

Can anyone please suggest me the best working concentration of Hoechst stain for neuroblastoma cell lines??

02 March 2016 5,964 1 View

What are the available tools & techniques to figure out a gene coding for a distinguishable phenotype in lizards ?

What are the available tools & techniques to figure out a gene coding for a distinguishable phenotype in lizards ?

11 December 2015 9,414 0 View

If two proteins run in sds page with different molecular weights, if both show bands with same intensity, does it mean they have same concentration?

If two proteins run in sds page with different molecular weights(having difference of 60 KDa),if both shows bands with same intensity,does it mean they have same concentration?

10 November 2015 6,879 3 View

Can anyone please suggest the name of any software or website useful for generating animated cartoon videos for explaining biological reactions ??

Please suggest the name of any user friendly free software or websites used for generating cartoon videos or animated pictures for explaining molecular reactions involving biological molecules and...

08 September 2015 7,439 0 View

After pelleting down the cells for plasmid isolation or protein purification , why did I find black substances along with the white pellets?

06 July 2015 6,815 5 View

Amount of a polyhistagged protein in an elutionbuffer having 30mM imidazole after an elution with a 350mM imidazole containing elutionbuffer is more?

Amount of a polyhistidine tagged protein in an elution buffer having 30mM imidazole just after an elution with a 350 mM imidazole containing elution buffer is significantly more.Can anyone please...

02 March 2015 6,800 9 View

In SDS PAGE, actin crosslinking doesn't show any significant increase in mass comparing actin bindings - any thoughts?

Can anyone help me to explain while running my protein samples in SDS PAGE, Actin crosslinking doesn't make any significant increase in molecular mass comparing actin binding protein samples.

07 August 2014 6,241 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View