What are the available tools & techniques to figure out a gene coding for a distinguishable phenotype in lizards ?
How can I estimate Kd value or any parameter showing the affinity of protein-protein interactions, If I have measurements(band intensities from SDS PAGE) only in linear range? Any methods, other...
08 September 2017 9,731 4 View
Can anyone please suggest me a detailed technique for collecting insect secretions in nano litre range?
02 March 2016 9,025 4 View
Can anyone please suggest me the best working concentration of Hoechst stain for neuroblastoma cell lines??
02 March 2016 6,024 1 View
If two proteins run in sds page with different molecular weights(having difference of 60 KDa),if both shows bands with same intensity,does it mean they have same concentration?
10 November 2015 6,936 3 View
Please suggest the name of any user friendly free software or websites used for generating cartoon videos or animated pictures for explaining molecular reactions involving biological molecules and...
08 September 2015 7,506 0 View
After pelleting down the cells for plasmid isolation or protein purification ,I found there are black substances along with the white pellets usually in the bottom part showing higher molecular...
06 July 2015 6,887 5 View
Amount of a polyhistidine tagged protein in an elution buffer having 30mM imidazole just after an elution with a 350 mM imidazole containing elution buffer is significantly more.Can anyone please...
02 March 2015 6,865 9 View
The recognition sequence for the restriction endonuclease AstWI is GRCGYC where R is any Purine and Y is any Pyrimidine base. In a truly random DNA sequence what will be the average distance...
11 December 2014 5,722 1 View
Can anyone help me to explain while running my protein samples in SDS PAGE, Actin crosslinking doesn't make any significant increase in molecular mass comparing actin binding protein samples.
07 August 2014 6,303 3 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View