After pelleting down the cells for plasmid isolation or protein purification ,I found there are black substances along with the white pellets usually in the bottom part showing higher molecular weight Can anyone please explain what these black substances are ??
If you mean E. coli spun down from culture, the black stuff is just junk from the growth medium. It doesn't affect your purification and you can safely ignore it.
If you mean E. coli spun down from culture, the black stuff is just junk from the growth medium. It doesn't affect your purification and you can safely ignore it.
I want to know what is the chemical composition of that black substance.
It may be just some dust. If you grow your E. coli in flasks with cotton or cellulose plugs, those may be just particles of cotton or cellulose. Though, cellulose plugs produce much less dust.
hey....it might be some dust particles..cotton fibers. some chances of any other kind of dye components.
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