Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..??

06 August 2016 1 6K Report

Hello friends, Can anyone please suggest a normal breast-associated fibroblasts (NAF) and Breast carcinoma associated fibroblast(CAF) cell line..??

One more query, I have HaCat cell line, can it be of any use in relation to above cell lines...??

S Ashraf Imam

Primary culture of breast cancer-associated fibroblasts are commercially available. However, they are not established cell lines.

Badges
Science topic

More Bikash Chandra Jena's questions See All

What is the best way to prepare delaminated Mxene?

We have Ti3C2Tx powder. Al has already been etched, and the method of etching is not known. The SEM image of the powder is attached. What is the best way to produce delaminated Mxene from this...

01 May 2024 4,130 0 View

What is the solution of the attached Bessel integral?

Can anyone suggest me some references to evaluate the attached integral for positive values of m, p, \mu, \nu, a, b, and c? I went through the book "Table of Integrals, series and products", but...

01 May 2024 8,703 0 View

Enrichment calculator can't detect docking output file in maestro ?

I performed a docking task but when I use enrichment calculator to generate ROC curve the output file are not in a format that is recognized by maestro. How can I solve this problem?

24 April 2024 7,843 1 View

Number of coordinates in coordinate file (EM.gro, 2171)does not match topology (topol.top, 30886)?

I am running a RNA ligand simulation on gromacs , i encountered a problem in which the number of coordinates in coordinate file (EM.gro, 2171) does not match topology (topol.top, 30886) file....

08 April 2024 4,660 2 View

Can we simulate RNA-ligand complex by using the software DESMOND ?

I am doing simulation of RNA-LIGAND complex with the help of software desmond but at last i only found the graph of ligand . I think desmond can't able to detect the RNA. please help me with this...

03 April 2024 6,421 1 View

Which filter paper is used for filtration of compounds mixed with silica from a TLC plate?

I got some bands of my compound in TLC. I want to get those compounds for further analysis means for quantification purpose. After scratching the band I have mixed with methanol in a conical flask...

29 March 2024 9,784 3 View

How to plot the dispersion band diagram for metal based photonic crystal using COMSOL?

I am learning the COMSOL software to study the band diagram of different photonic crystal structures. For a dielectric-based photonic crystal, only the k parameter sweep is needed to study the...

25 March 2024 3,514 1 View

Which are the critical parameters which basically affect the floating lag time?

We are basically preparing floating tablets and facing problems for minimizing the floating lag time. Please suggest.

13 March 2024 4,693 0 View

How to do refinement of Single crystal using Rietveld refinement ? Is it same as polycrystalline samplerefinement?

Please reply me details about the single crystal Rietveld refinement.

23 November 2023 8,365 1 View

How can i convert aerogel into powder for xps. i treid to grind it but i couldn't?

i need powder form of my aerogel for xps analysis, how can i do that.

04 September 2023 2,059 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View