Can anyone please provide me with a plasmid vector with GFP tag ?

More Himangshu Boruah's questions See All

What are the most common protocols to quantify arsenic using HG-AAS?

I want to determine arsenic (both arsenite and arsenate) concentration in water, soil and rice samples using HG-AAS. Kindly suggest some routine methods.

07 August 2018 9,116 1 View

How can I quantify nitrogenase activity without using Gas Chromatography in bacteria?

Nitrogenase activity, Microbiology.

09 October 2014 642 6 View

What is the information obtained from SAED pattern of nanopoarticles?

The SAED pattern for the nanoparticles.

04 May 2014 2,980 47 View

What information is obtained from SAED pattern of nanopoarticles?

04 May 2014 6,533 3 View

How XRD peaks change with different orientation of planes in nanopoarticles?

What is the relation between XRD peaks and orientation of planes?

04 May 2014 1,200 13 View

How does the size, shape, and concentration of nanoparticles depend on the concentration of reducing agent?

When nanoparticles are synthesized by chemical reduction, how does the size, shape, and concentration of the nanoparticles change with the change in concentration of reducing agent?

04 May 2014 7,432 8 View

Is there any definite rule to determine how polymer nanocomposite strength depends on the nanoparticles concentrations?

I want to know whether there is any relation between nanoparticle concentration and the strength of the polymer nanocomposite. I have used starch as polymer and ZnO nanoparticles. Does shape and...

04 May 2013 4,908 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Hakim Samai

mcherry vectors are the best for me, already used it

Joanne Kamens

We have many mCherry and 1000's of FP vector backbones www.addgene.org. Please email [email protected] for suggestions. The more you tell our scientist tech team what you need, the better they can help you select a plasmid.