I want to see the expression of a gene using this vector in E. coli.
mcherry vectors are the best for me, already used it
We have many mCherry and 1000's of FP vector backbones www.addgene.org. Please email [email protected] for suggestions. The more you tell our scientist tech team what you need, the better they can help you select a plasmid.
I want to determine arsenic (both arsenite and arsenate) concentration in water, soil and rice samples using HG-AAS. Kindly suggest some routine methods.
07 August 2018 9,178 1 View
Nitrogenase activity, Microbiology.
09 October 2014 712 6 View
The SAED pattern for the nanoparticles.
04 May 2014 3,048 47 View
What information is obtained from SAED pattern of nanopoarticles?
04 May 2014 6,583 3 View
What is the relation between XRD peaks and orientation of planes?
04 May 2014 1,280 13 View
When nanoparticles are synthesized by chemical reduction, how does the size, shape, and concentration of the nanoparticles change with the change in concentration of reducing agent?
04 May 2014 7,474 8 View
I want to know whether there is any relation between nanoparticle concentration and the strength of the polymer nanocomposite. I have used starch as polymer and ZnO nanoparticles. Does shape and...
04 May 2013 4,957 10 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View