Thank you very much for your kind response. We want to quantify nitrogenase activity of free living as well as symbiotic nitrogen fixing bacteria.Please let us know.
Acetylene Reduction Assay is used to measure Nitrogenase Activity in microbiological samples. Exposure to acetylene inhibits nitrogenase activity in many legume symbioses.cetylene reduction assay. Nitrogenase reduces acetylene (C2H2) to ethylene (C2H4), and the ethylene.
If have a cyclotron and can work fast you can determine the reduction of N13, which has a half life of less than ten minutes. Confident it has been done.