Can anyone please explain the role of miRNAs during type-2 diabetic condition?

More Mayilvanan Chinnaiyan's questions See All

DNA isolation methods - DNA yield is lesser in micro wave method then in salting out method and commercial kit method. Why?

We are isolating DNA from wax embedded Squamous cell carcinoma (SCC) tissues. We take tissue sections and isolate the DNA from that. We following 3 methods for a comparison study. Those methods...

10 November 2017 9,809 0 View

During DNA isolation how do u make sure that you have isolated only the DNA not RNA?

While isolating DNA how do we make sure that we have isolated only the DNA at the end not RNA? At which step the DNA alone get separated and which buffer and composition is used for that... Thank You

09 October 2017 794 5 View

Can any one tell me the important genes involved in adipogenesis regulation during type-2 diabetic condition?

I would like to know the important genes involved in adipogenesis regulatory mechanism during high fat fed condition in Wistar rats or during type-2 diabetic condition...

04 May 2014 3,883 4 View

How do you differentiate Wistar and SD rats? Through any morphological differences?

When you look at both rats they are albino and hard to differentiate. So my question is what are the peculiarities does both has?

02 March 2014 9,400 1 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Is there a good "no frills" search tool for highlighted text?

I am looking for a no frills search tool that will find highlighted text in pdf and epub files. I just want something that will just take me to the next highlighted word or phrase in the way a...

07 July 2024 190 5 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

19 June 2024 4,210 3 View

How to mimic diabetic conditions in cell culture?

Is there a protocol to establish diabetic conditions in pre-osteoblasts cell culture?

18 June 2024 9,306 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

IL-2 for PBMCs ex-vivo stimulation, best Concentration?

Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...

16 June 2024 928 1 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Vishal Sarsani

Mayilvanan,

Many recent studies show that expression of microRNAs can affect insulin secretion which is related to type-2 diabetes

Please look into following papers :

http://diabetes.diabetesjournals.org/content/60/7/1825.full

http://www.ncbi.nlm.nih.gov/pubmed/21118127

Mayilvanan Chinnaiyan

Thank you so much..

Mojtaba Parvizi

Many effect could be discussed here. Effect on insulin, vessels ans etc. It is depend on your research area and focuses.

Ya right now I am working in high fat diet induced insulin resistance, on which I would like to know the miRNA aspects in glucose homeostasis in skeletal muscle and adipogenesis regulation. Any one, can you help me in that aspects.