How to start work on circulating miRNA - whole blood of PE patients?

Dear Friends, I need guidance in initiating work on circulating miRNA - whole blood in PE patients. What are the requirements and how to collect the blood Required Protocols...

08 September 2019 7,835 4 View

How to lyophilize aqueous plant extract by conventional methods?

I have a plant extract which needs to be concentrated and I do not have lyophilizer. Can anyone please suggest me any conventional method.

11 December 2013 7,389 0 View

Help with Gene expression studies in primary hippocampal cell cultures and brain tissue

I am looking for the protocols in order to do standardizing expression of the inflammatory genes at gene level, when induced with some neurotoxins and I am unable to decide which reagents i...

10 November 2011 6,027 2 View

best media suitable for Rat Hippocampal neurons for both Organotypic and primary cell cultures

I am wanting to start work on organotypic and primary cell cultures of post natal rat hippocampus and i am not sure which media promises good results, should i go with MEM+ FBS or DMEM+FBS or...

10 November 2011 7,683 5 View

How and why does the compound action potential change as the stimulus intensity increases?

please let me know the relation betn Action potential and stimulus intensity

09 October 2011 5,570 0 View

What affect does the anesthetic have on the compound action potential ?

Mechanism of action of Anesthetics on generation of action potential and conduction

09 October 2011 1,578 1 View

Despite presenting a degree of organization, neurons in the hypothalamus do not present lamination as in the optic tectum. Why do you think the differ...

I want to learn more of the layering and purpose of layer in two different regions of brain

09 October 2011 4,563 3 View

Why is the stimulus voltage required to reach threshold different with different stimulus duration?

Factors governing the threshold potential and duration of stimulus

09 October 2011 7,548 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View