Can anyone one help with W20-17 stromal cells?

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How to start work on circulating miRNA - whole blood of PE patients?

Dear Friends, I need guidance in initiating work on circulating miRNA - whole blood in PE patients. What are the requirements and how to collect the blood Required Protocols...

08 September 2019 7,922 4 View

How to lyophilize aqueous plant extract by conventional methods?

I have a plant extract which needs to be concentrated and I do not have lyophilizer. Can anyone please suggest me any conventional method.

11 December 2013 7,490 0 View

Help with Gene expression studies in primary hippocampal cell cultures and brain tissue

I am looking for the protocols in order to do standardizing expression of the inflammatory genes at gene level, when induced with some neurotoxins and I am unable to decide which reagents i...

10 November 2011 6,122 2 View

best media suitable for Rat Hippocampal neurons for both Organotypic and primary cell cultures

I am wanting to start work on organotypic and primary cell cultures of post natal rat hippocampus and i am not sure which media promises good results, should i go with MEM+ FBS or DMEM+FBS or...

10 November 2011 7,779 5 View

How and why does the compound action potential change as the stimulus intensity increases?

please let me know the relation betn Action potential and stimulus intensity

09 October 2011 5,671 0 View

What affect does the anesthetic have on the compound action potential ?

Mechanism of action of Anesthetics on generation of action potential and conduction

09 October 2011 1,666 1 View

Why is the stimulus voltage required to reach threshold different with different stimulus duration?

Factors governing the threshold potential and duration of stimulus

09 October 2011 7,638 0 View

Despite presenting a degree of organization, neurons in the hypothalamus do not present lamination as in the optic tectum. Why do you think the differ...

I want to learn more of the layering and purpose of layer in two different regions of brain

09 October 2011 4,656 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View