How to start work on circulating miRNA - whole blood of PE patients?

Dear Friends, I need guidance in initiating work on circulating miRNA - whole blood in PE patients. What are the requirements and how to collect the blood Required Protocols...

08 September 2019 7,835 4 View

Can anyone one help with W20-17 stromal cells?

Dear Friends I am looking for W20-17 mouse stromal cells for my in vitro studies, can anyone help me by providing this particular cell line. Your help will be highly...

31 December 2018 6,528 2 View

How to lyophilize aqueous plant extract by conventional methods?

I have a plant extract which needs to be concentrated and I do not have lyophilizer. Can anyone please suggest me any conventional method.

11 December 2013 7,389 0 View

Help with Gene expression studies in primary hippocampal cell cultures and brain tissue

I am looking for the protocols in order to do standardizing expression of the inflammatory genes at gene level, when induced with some neurotoxins and I am unable to decide which reagents i...

10 November 2011 6,027 2 View

best media suitable for Rat Hippocampal neurons for both Organotypic and primary cell cultures

I am wanting to start work on organotypic and primary cell cultures of post natal rat hippocampus and i am not sure which media promises good results, should i go with MEM+ FBS or DMEM+FBS or...

10 November 2011 7,683 5 View

What affect does the anesthetic have on the compound action potential ?

Mechanism of action of Anesthetics on generation of action potential and conduction

09 October 2011 1,578 1 View

Despite presenting a degree of organization, neurons in the hypothalamus do not present lamination as in the optic tectum. Why do you think the differ...

I want to learn more of the layering and purpose of layer in two different regions of brain

09 October 2011 4,563 3 View

Why is the stimulus voltage required to reach threshold different with different stimulus duration?

Factors governing the threshold potential and duration of stimulus

09 October 2011 7,548 0 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Can you apply graph theoratic functional connectivity analysis to study cognitive processes under external stimuli?

Can functional connectivity analysis using Graph Theory (Hub-ness, clustering coefficient, etc) be applied to study cognitive processes? I felt most of the analysis to study the brain after...

25 January 2021 4,575 3 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

What are some recent publications showing the predictor power of resting state brain acitivity for tasks done outside the MRI scanner?

Looking for recent articles (2018-2020) that showed resting state brain activity can predict cognitive task performance (e.g., attentional and executive functioning tasks) done in the lab AFTER...

09 January 2021 2,310 2 View

What are the essential elementts of a Nature Neuroscience Paper?

What would land a neuroscience paper in Nature Neuroscience? What is the minimum a paper should have to pass the editorial scrutiny in Nature Neuroscience or the journals around the same cadre?...

01 January 2021 1,510 2 View