I am looking for the protocols in order to do standardizing expression of the inflammatory genes at gene level, when induced with some neurotoxins and I am unable to decide which reagents i should use and etc, Kindly help me.
I am not working on this field. But, according to my limited knowledge, Realtime qPCR could be used to judge imflammatory genes (i.e. IL-6, TNF-alfa) changes in expression levels.
You may use corticosterone, MPP+, Amyloid beta, Glutamate, Peroxide, hydroperoxide,... as neurotoxins and you can check Interleukin, Cox2, TNF-alfa activities by RT-PCR/ westernblotting,...