How to start work on circulating miRNA - whole blood of PE patients?

Dear Friends, I need guidance in initiating work on circulating miRNA - whole blood in PE patients. What are the requirements and how to collect the blood Required Protocols...

08 September 2019 7,835 4 View

Can anyone one help with W20-17 stromal cells?

Dear Friends I am looking for W20-17 mouse stromal cells for my in vitro studies, can anyone help me by providing this particular cell line. Your help will be highly...

31 December 2018 6,528 2 View

Help with Gene expression studies in primary hippocampal cell cultures and brain tissue

I am looking for the protocols in order to do standardizing expression of the inflammatory genes at gene level, when induced with some neurotoxins and I am unable to decide which reagents i...

10 November 2011 6,027 2 View

best media suitable for Rat Hippocampal neurons for both Organotypic and primary cell cultures

I am wanting to start work on organotypic and primary cell cultures of post natal rat hippocampus and i am not sure which media promises good results, should i go with MEM+ FBS or DMEM+FBS or...

10 November 2011 7,683 5 View

How and why does the compound action potential change as the stimulus intensity increases?

please let me know the relation betn Action potential and stimulus intensity

09 October 2011 5,570 0 View

What affect does the anesthetic have on the compound action potential ?

Mechanism of action of Anesthetics on generation of action potential and conduction

09 October 2011 1,578 1 View

Despite presenting a degree of organization, neurons in the hypothalamus do not present lamination as in the optic tectum. Why do you think the differ...

I want to learn more of the layering and purpose of layer in two different regions of brain

09 October 2011 4,563 3 View

Why is the stimulus voltage required to reach threshold different with different stimulus duration?

Factors governing the threshold potential and duration of stimulus

09 October 2011 7,548 0 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View