Can anyone indicate a cosmid vector available from AddGene for gene expression in Pseudomonas or Gram-negative bacteria?

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Can anyone indicate a cosmid vector available from AddGene, similar to pJRD215, for gene expression in Pseudomonas and other Gram-negative bacteria?

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Benjamin Lundgren

Not sure about cosmid, but the pBBR1MCS plasmid series works great in Pseudomonas. It is pretty low copy, uses the generic lac promoter for expression, and there are derivatives for various antibiotic markers (carb, gent, tet, etc.). You can check out the original study, and if you need any of these plasmids, I can potentially send them to you.

Edmar Oliveira-Filho

Benjamin,

We are already using the pBBRMCS1 vectors.

Many thanks for being available to send them!