Is there anyone that is developing research about the influence of glial scar on neuroregeneration?

More Manoel Baldoino Leal Filho's questions See All

How to count and standarize plankton on a chamber kolkwitz?

How use this chamber and standarize the data

17 July 2024 1,844 2 View

Must a theory be falsifiable to contribute to good science?

I've been wondering for some time - must a theory be falsifiable to contribute to good science? What would be the role, if any, that non-testable theories play in biology, especially regarding...

17 June 2024 4,809 10 View

Does anyone knows how to convert an h5ad file into rds file, using an python script?

09 April 2024 1,520 2 View

How to design a boostrapping test for dfferential expression?

Given a set samples e.g. n=1000 patients, 500 cases and 500 controls, but only 30% men and 1% smokers, the proposal to take heterogeneity into account would be to use bootrapping b=100, and...

27 December 2023 3,727 1 View

How to use multiomics for clinical trials?

Association of the effect of the medication on the disease (efficiency), but also the effect of the drug the body in general (side effects). Furthermore, the association of the effect of the...

26 December 2023 8,566 2 View

Do viruses promote the evolution of their hosts by acting as vectors of horizontal gene transfer?

Viruses can transfer copies of genes from their hosts to other host organisms, a process called horizontal gene transfer. Part of the non coding genes of a given organism wich is transmited...

19 December 2023 4,569 1 View

Which medium it's the best for growing Leishmania mexicana?

25 November 2023 7,676 3 View

What are the availble computational tools to down-sample Diffusion Weighted Images (DWI)?

In order to analyse the effect of data manipulation on DWI images, it is commonly interesting to understand the effect of number of gradients. When a original DWI data is acquired with a...

23 November 2023 2,560 2 View

What are the available toolkit for Diffusion Tensor Imaging simulation?

I am wondering if there are any available tools that can provide a simulation of Diffusion Tensor Imaging for a controlled set of studies. Are there options with canonical geometries such as...

17 November 2023 3,409 1 View

Is this a Foraminifer?

I want to ask you if this is a foram or not. If it is, what genre or even specie we're talking about? Please. This is a sample collected in the East Pacific Ocean, specifically in the coast of...

18 September 2023 6,667 4 View

Neuronal Media: Can I mix all supplements (B27, N2, GDNF/BDNF) into one "master" aliquot to freeze?

For neuronal media, there are some factors such as B27, N2, GDNF, and BDNF that are best added fresh into the basal media the day of. Instead of having a bunch of aliquots of each...

04 April 2024 8,733 2 View

Why is my IBA1 antibody not working?

I have tried 4 IBA1 antibodies and cannot seem to get good staining. Mice were perfused/fixed with PBS/4%PFA. Brains were placed in PFA overnight, and then moved to 30% sucrose and frozen in OCT...

19 February 2024 2,835 5 View

What is the technical difference of CAPS and SCAR markers in genetic engineering?

Genetic Engineering. Markers.

03 February 2024 4,012 0 View

Can anyone share insights into the role of non-coding RNAs in neural development and their potential implications for neuroregeneration therapies?

Non-coding RNAs (ncRNAs) are commonly known as RNA molecules that are not involved in the protein-coding process but play crucial regulatory roles in various cellular processes, including neural...

28 December 2023 8,159 2 View

Hello, I am a 5th-year medical student. I want to initiate research in the field of neuroregeneration after injury ?

Hello, I am a fifth-year medical student. I want to initiate research in the field of neuroregeneration after injury. However, I am facing difficulties in identifying a starting point for my...

10 August 2023 3,306 1 View

How to increase GFAP Antibody Penetration on Organtopic Cultures?

In my project, I aim to use immunohistochemistry to stain astrocytes in organotypic slices. For now, I have tested two antibodies, both of them for GFAP: GFAP rabbit from Sigma-Aldrich (G9269);...

14 February 2023 939 2 View

Where can I do some of the Aging Markers for my Research?

Dear Colleagues, We are trying to set up the Research on Aging, and we are interested in doing some of the markers. I need help with finding who would be able to this for us? Which lab or...

10 January 2023 5,079 0 View

What does GFAP mark ?

Hello, GFAP means Glial fibrillary acidic protein so it should mark every glial cell ? Because I've seen many articles using it as an astrocyte marker only. Thank you for your responses !

10 July 2022 5,477 3 View

Problems for isolation DRG?

Hi! Lately I have been trying to isolate and culture DRG from mice, but I am having some issue with it. I am following the ptocol of C. Sokol (2021); birefly: First, I coat the plate with a...

10 March 2022 6,812 5 View

What would be the possible reason of getting nonspecific band using SCAR markers?

Hi Folks, I am recently getting some weird nonspecific bands (I suppose to get only one) using SCAR markers while using the same protocol, PCR program and primers, does anyone possibly knows what...

14 March 2021 9,205 2 View