I want to inoculate MCF7 or T47D cell lines after female albino mice irradiation. I read that it requires E2 pellet and that cell lines should be injected with Matrigel. Could you send me your recommendations about these points and the time needed for tumor to appear for these cell lines (both subcutaneous and orthotopic)?
We've published our general procedures for xenografts with MCF-7 (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/mcf7-xenograft-model/) and T-47D (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/t-47d-xenograft-model/). Estrogen pellets and matrigel are often necessary, and I haven't heard of researchers being able to record decent growth curves without them. Tumor growth is pretty stable, and rises quickly to the point where studies last only around a month.
Dear Doaa, actually better to use 4T1 cell line for murine breast cancer induction. However, I used MCF-7 in mice without matrigel and the cancer appeared after 10 days, depend on how many cells you inject? and viability of the cells. TQ
Thanks a lot for the reply. Can you tell me about the best cell count to start with.
Hi Doaa,
We inject 5 million mcf7 cells in saline (subcutaneous) into nude mice and get tumour formation at 2 weeks. The mice are first injected with the estradiol pellets one day before the cells are injected. For us, if we do not inject the pellets the tumours take much longer to grow and are much more variable in the time to form tumours.
Hope it helps,
Jacob
Cells in the system of multicelular organism from positions of non-linear dynamics
V. A. Kotolupov, V. V. Isaeva
I had tried 1 million cells with metrigel and gave the E2 pellet at the same time. We got the tumor within 10 days.
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