What are the validation timelines for quantative real time PCR and western blotting results, is it 24 hours for qpcr and 48 hours for western blotting if so! Why then!?
that is a wide broad questions. obviously depends on experiments conditions and the question that you want to answer. Normally, we do time course or dose response for a 24, 48, 72h.
It depends on your treatment efficiency. Researchers conducts 24, 48, and 72 hours of treatment and if required do it for a long time. Technically, treatment conditions for both PCR and Western experiments should be of the same time period. However, sometimes impact on protein expression may need more time.