How many animals should be used in the study of drug efficacy in a 1,2-Dimethylhydrazine colon cancer rat model? When should I start infused medicine? What markers in blood should I look for? How to determine the degree of effectiveness?
Dear , for animal groups, better to have at least 6 animals per group (control negative, cancer control, treatment low dose, treatment high dose, and control positive). After 1 week of injection, you need to sacrifice one animal in cancer control group (better to do preliminary or pilot study for this case), send colon samples for H&E to confirm cancer induction. Then you can start treatment for how long you want (1-4 months, depends). Blood bio markers like Beta Catenin is very important in your case. Then at last you can do PCR, WB, IHC, H&E to confirm effectiveness. Wish you all the best, TQ
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