How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Does anyone use the ISE electrodes (ammonia, nitrate etc.) to characterise soils samples and plants extracts? What are advantages & disadvanteges?

I will have numerous soil samples/rice rhizosphere sample to characterise as part of the rice microbiome study. I was told that there are ISE electrodes that can be used to measure ammonia,...

19 July 2020 2,066 3 View

Which anti: GRP94 antibody do you recommend?

I am working with C.elegans strain which expresses H.s. GRP94 protein. I am looking for proper antibody to detect GRP94. Do you have some recommendation or suggestions?

13 September 2016 7,214 1 View

Do I need phylogenetic correction to my analyses when I have in data base 11 species?

Dear all, I am performing different analyses (ANOVA, ANCOVA, correlations etc.) and I am not sure if I need phylogenetic correction. On my dataset I have 11 closely related species from Carabidae...

22 November 2015 5,406 12 View

I'm looking for cell dye, which changes it's colour when entering the cell. Can anyone help?

18 October 2015 6,368 3 View

Does anyone have experience with polytypes of CdI2 and their structural calculations?

Or maybe know someone who has experience in this field?

08 February 2015 4,806 3 View

How can I analyse fluorescent images of Double/triple positive cells?

Hi I am using a Columbus for fluo images analysis. I need to define double or triple positive cells. My nuclei are stained with Hoestch (all cells), Sytox (dead cells only) and FITCs (apoptotic...

05 January 2015 3,830 4 View

Does anyone know how to soften the eyeball for histology (paraffin technique)?

02 December 2014 7,688 3 View

Can anyone help me to improve my Lipase assay Kwon and Rhee?

Could anyone help me to improve my lipase assay. I need to study the lipase activity produced by Bacillus saliphilus isolated from alkaline waste lime. I try to apply the method by Kwon and Rhee....

16 September 2014 9,717 29 View

Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

Detection range around 10-20pg/ml. I have used one but it didn't even detect UCN2 added to the wells. Thanks.

20 May 2014 6,892 5 View

If you haven't computer? how you show the photons to pupils?

Educational Process

01 March 2021 1,827 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Cell culture compatible glue/sealant?

Hello We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and...

01 February 2021 6,886 3 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

Can data collected through participant observation be interpreted through Interpretative phenomenology?

I have just started to read about phenomenology and hence quite a beginner. I have observed hospital interactions between doctors and patients, noted down the conversations, added the non-verbal...

14 January 2021 3,452 3 View

Johnson et al., 2006 Geology of South Africa?

I am wondering if there is a digital (PDF) version of this book available or if anyone is willing to share this work. It is no longer available from GSSA (Geo Soc. S. Afr) nor does my library hold...

13 January 2021 5,571 2 View

Do you think that genetic inheritance or the environment is more influential in the development of substance abuse?

Do you think that genetic inheritance or the environment is more influential in the development of substance abuse ?

04 January 2021 1,587 3 View

What is the current status of black female police officers?

I am a doctoral candidate at Walden University. My dissertation is centered on the lived experience of black female police officers and their perspective of excessive use of force administered...

29 December 2020 8,452 1 View