I am trying to find a suitable model to study certain questions. 

A. Dynamics of molecular subcellular localisation under different conditions.

B. Calcium Dynamics.

To study these two objectives under live- imaging conditions, I would like to know which system will be better. Hippocampal organotypic slice cultures or hippcampal dissociated neuronal cultures from embryonic stage. 

If hippocampal organotypic slice cultures are good model for these objectives, is it easy to observe such subcellular localisation dynamics of molecules under live imaging/ after PFA fixation and immunostaining in slice cultures. I am just doubtful because of the tangling and very close positioning of the neurites in the slices which might be a problem for analysis in slices if there is any change in subcellular localisation of the molecules after certain treatments.

Anyone with experience with this line of work, please help.