Has anyone experience with miRNeasy Serum/Plasma Kit in samples stored at -80oC for more than 1 year? Any Problems?
If the samples were continuously frozen, they should be stable for years. I would recommend the miRVana Kit (Ambion), its quite efficient for microRNA extraction from serum/plasma. Serum/Plasma volume between 170 - 400 µl is recommendet for microRNA analysis.
If the samples were continuously frozen, they should be stable for years. I would recommend the miRVana Kit (Ambion), its quite efficient for microRNA extraction from serum/plasma. Serum/Plasma volume between 170 - 400 µl is recommendet for microRNA analysis.
Our group has extracted miRNAs from plasma too and first we stored the samples at +4°C, and one hour after we stored at-20°C. One day later we stored at -80°C. We do this to avoid the formation of ice crystals in the samples. We are using the miRvana invitrogen kit
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