If the samples were continuously frozen, they should be stable for years. I would recommend the miRVana Kit (Ambion), its quite efficient for microRNA extraction from serum/plasma. Serum/Plasma volume between 170 - 400 µl is recommendet for microRNA analysis.
If the samples were continuously frozen, they should be stable for years. I would recommend the miRVana Kit (Ambion), its quite efficient for microRNA extraction from serum/plasma. Serum/Plasma volume between 170 - 400 µl is recommendet for microRNA analysis.
Our group has extracted miRNAs from plasma too and first we stored the samples at +4°C, and one hour after we stored at-20°C. One day later we stored at -80°C. We do this to avoid the formation of ice crystals in the samples. We are using the miRvana invitrogen kit