Dear Arun,

please rewrite your question and tell us if you watch and examine in vivo at first and then in vitro after the staining with acridine orange.

What a kind of cells are you using (monolayers /Fibroblastes, HeLa cells ....)

Details about vital staining or fixation media are needed.

On other part , what is the source of your mitochondrial damage fragments. Please mor infos for a good answer.

JRG