Can anyone help with antibody pairs for 2-plex fluorescence WB?

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Jordan R. Yaron

You could try a company like ECM Biosciences to look for antibodies. They specialize in phospho-specificity.

Liaque mohammed Latif

One way is to use the antibody of interest with its respective anti Phospho antibody in which case you already have the best supplier in the field of such antibody pairs, CST. Alternatively, as i have done, i use anti GAPDH, Anti Beta actin or anti Alpha Actin, which can all be purchased from Sigma, raised in either mouse or Rabbit. These can be used as loading controls and you use a Anti Phos antibody of interest. This of corse will not be suitable if you anticipate effects on the un-phoshporylated expression of the protein. I find this to work fine for short drug exposures.