I attached 5 pictures to help with identification. THANK YOU... I am grateful.
This appears to be a fungus in the genus Alternaria - Alternaria species are common saprophytic and occasionally plant pathogenic fungi.
This is surely a fungus. But given data is not sufficient to guess the genus level exactly. Most probably this is a Dothideomycete with muriform spores. There are lots of families in order Pleosporales have this kind of spores.
Hi Kimberly,
The pictures are low quality, but if the spores at the top are ascospores produced within fruiting bodies with abundant setae (pictures just below), the fungus might be a species of Pyrenophora, or a related genus.
Nice fungus, will you send more photos?
The picture at the bottom doesn't look like fungal elements, but simply fibers of plant material
Cheers,
Hugo
Yeah, the spores do look like Alternaria, but it is hard to tell based on the black pics provided. DNA analysis will tell you for sure, but there are also a lot of good sources on the Internet. Type in Alternaria and look under the images. You should find lots of pics that match your microscopic images. Best of luck!
Dear Kimberly, this is fungi genus Alternaria. This are macroconidia specific for Alternaria spp.
The last picture not present fungal elements, this is "artifact" it means non-medial elements, from stain or swab.
Alternaria is is good first guess, but not definitive. It could be a member of a number of other genera such as Ulocladium, Embellisia, Parapithomyces, Amoenomyces, etc. (see The Genera of Hyphomycetes, by Seifert et al.) You really need to see CLEARLY the conidia, arrangement of conidia, conidiogenesis, conidiophores and arrangement of conidiophores. Now, getting to the correct species requires consulting a specific monograph of the correct genus (available in the journal literature.)
Of course the modern method of identification is to send it to a service for genetic analysis. This is not completely reliable because it relies on the existing database (which may be wrong) for comparison, but it can help if it backs up the visual microscopic identification.
It is a fungus, however data is not sufficient for genus name, but especially the first picture appears Alternaria knowing plant pathogenic fungi.
It is a fungus. To determine the genera you may do sequencing of ribosomal genes (Nuclear ribosomal internal transcribed spacer (ITS), 18S rRNA, etc) and/or mitochondrial genes (cytochrome b for example, which may or may not give you the species level depending on the databases available or positive controls). If you have controls for especies of Alternaria (which seems to be the most likely genera) then you can use whatever information is available on GeneBank to support your results.
The dematiaceous colony and muriform, phaeoid condia are consistent with Alternaria
Definitely not Thielaviopsis. May be Pleospora or Chaetomium, both of which have some species that have an Alternaria anamorph form, but without evidence of the perfect forms, I agree with Dennis Babel that colony and conidia are consistent with Alternaria. Many genera have an Alternaria anamorphic form. See "The Genera of Hyphomycetes" by Seifert et.al. 2011, p. 75.
Can you do a slide culture of this isolate and send photomicrograph of conidial pattern which will helpful in identifying the isolate.
this fungus species has not a beak then it can not be Alternaria spp
These spores resembles Graphyllium spp. But visible setae that covered ?ascocarps suggests other fungus.
Yes it is a fungus. It may belong to Alternaria species. Microscopic studies will help you.
The spores in your pictures seemed as Alternaria but it also resemble Cochliobolus
compare this spores with the pictures given in Fungal families of the world by p.f cannon
Dear Kimberly, can you take more clear pictures? Pictures with adjusted focus and clear view are helpful. It seems to be Alternaria. However, higher resolution and higher magnification will be more helpful.
It seems belong to Alternaria species.But Most probably this is a Dothideomycete with muriform spores.That´s what I think !!
Nadeem... thank you, thank you, THANK YOU... for helping me and my family!!!! Ever since March (when I first posted those pictures), I have been reading and reading and reading, trying to understand the world of FUNGI... Goodness there are SO MANY SPECIES!!!!! And they are ALL so COMPLEX!!! Months passed... and then I started realizing that I was "barely biting a crumb off the cookie." Plus, it was really hard for me to read about and LOOK at something that you really, deep down, just want to FORGET ABOUT FOREVER!!!!! My family and I have been suffering from our "infection/contamination" for over four years now and I'm ready for it to be GONE!!!! And since no doctor in Tennessee has been of ANY HELP to us, I decided to start posting again on this website, and ask for help... This is where all the EXPERTS are, and you guys know WAY more than any of those doctors I saw.
So here are my next questions... (they are in response, Nadeem, to YOUR response from yesterday)...
What appendages did you mean? On which structure of the fungus would they be?
Forever grateful, Kimberly :)
Iam sure that it is not Pestalotia even it is not Alternaria sps. Because Pestalotia sps have Conidia which are multi-celled with usually three darkly pigmented center cells and clear pointed end cells.diagnostic feature is the two or more clear, whisker-like appendages arising from the end cell. even it doesnt seems to be Alternaria . What is the source of isolation ?
Devadatha,
Do you mind if I send you a few more pictures? Your time and help with this is so greatly appreciated, to me and my family.
Thank you,
Kimberly
Yeah sure i will try my best to identify them. You can send the pics and spore size also to my mail.id
If you could write the description of the conidia, the identification would have become bit easier.
if no longitudinal septa are there then it goes near to Curvularia
Alternaria, chetomium or even Hortae wernickie possible kindly provide from which sample and other with this information we can tell you the exact one
N° 1 is Pithomyces sp. Concerning the other pictures it is not possible to say; more details are needed
Those pictures are not clear.
for morphological identification needs to check some characteristics such as color and form of colony, microscopic characteristics such as size and form of conidia and conidiophores, septate or aseptate and more details.
What is the source of these fungi?
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any way
If you want to do correct identification, you can send your fungal samples to me or an expert (mycologist) in your area for identifying.
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