Gulnaz Bashir

11 Questions 38 Answers 0 Followers

Questions related from Gulnaz Bashir

Has anybody used filmarray by Biomerieux for syndromic diagnosis?

Kindly share our experience with film array by BioMerieux for syndromic diagnosis using Meningitis/encephalitis, respiratory, GIT panel

21 April 2018 7,358 1 View

Is anybody woking on Colorimetric methods for diagnosis of TB?

I am currently working on colorimetric methods using resazurin and malachite green for diagnosis of TB. I am facing a problem of contamination with aerobic spore bearers after decontamination and...

01 December 2017 313 2 View

Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What...

04 November 2014 5,112 4 View

Can infection with mycobacteria give a false positive SAT for Brucella abortus.

A patient with a H/O laparoscopic hernioplasty with a mesh was investigated for PUO. His culture is negative for Brucella on three separate occasions but SAT positive for Brucella abortus and...

11 October 2014 1,455 3 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

I have recently purchased a new electrophoresis unit. On electrophoresis in 1 and 2% agarose, gel bands of same size on the electrode side move slowly giving the whole thing a slanting look. The...

08 October 2014 3,276 11 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

01 July 2014 4,262 4 View

Regarding Lyme disease, what are the diagnostic modalities available and their source, to rule out Borrelia burgdorferi as a cause of morphea?

As above.

22 May 2014 4,882 8 View

What is the cheapest and most rapid method of confirming MTB from BacTalert bottles giving a positive flag?

Can cording seen on AFB staining from the bottle be used as a direct confirmatory test for identification of MTB?

17 April 2014 4,938 7 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

15 April 2014 3,743 2 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

12 April 2014 9,257 30 View

What is the best way to preserve M.tuberculosis?

I have tried to preserve M. tuberculosis in glycerol and kept at -20C. On subculturing on LJ the recovery has not been good. Also strains die on repeated subculturing on LJ. Can anybody give a...

20 November 2013 5,007 5 View