Gulnaz Bashir

14 Questions 44 Answers 0 Followers

Questions related from Gulnaz Bashir

Has anybody used filmarray by Biomerieux for syndromic diagnosis?

Kindly share our experience with film array by BioMerieux for syndromic diagnosis using Meningitis/encephalitis, respiratory, GIT panel

21 April 2018 7,401 1 View

Is anybody woking on Colorimetric methods for diagnosis of TB?

I am currently working on colorimetric methods using resazurin and malachite green for diagnosis of TB. I am facing a problem of contamination with aerobic spore bearers after decontamination and...

01 December 2017 357 2 View

Any body having experience in using malachite green for TB diagnosis?

I'm trying to use malachite green for detection of MTB in samples. Many samples are showing decolourization at the time of sample inoculation only. Does pH have to do any thing with it?

29 November 2016 6,483 3 View

How commonly do you see Nocardia spp. in urine?

In case you recover it on culture, what should be the further mode of action.

09 March 2015 3,140 3 View

Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What...

04 November 2014 5,145 4 View

Can infection with mycobacteria give a false positive SAT for Brucella abortus.

A patient with a H/O laparoscopic hernioplasty with a mesh was investigated for PUO. His culture is negative for Brucella on three separate occasions but SAT positive for Brucella abortus and...

11 October 2014 1,497 3 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

I have recently purchased a new electrophoresis unit. On electrophoresis in 1 and 2% agarose, gel bands of same size on the electrode side move slowly giving the whole thing a slanting look. The...

08 October 2014 3,338 11 View

Which is the best method of identification and DST for MOTT/ NTM?

Is anybody doing DST and identification of NTMs in India. Want to get DST of a clinically significant NTM done urgently.

22 August 2014 5,976 8 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

01 July 2014 4,299 4 View

Regarding Lyme disease, what are the diagnostic modalities available and their source, to rule out Borrelia burgdorferi as a cause of morphea?

As above.

22 May 2014 4,919 8 View

What is the cheapest and most rapid method of confirming MTB from BacTalert bottles giving a positive flag?

Can cording seen on AFB staining from the bottle be used as a direct confirmatory test for identification of MTB?

17 April 2014 4,973 7 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

15 April 2014 3,788 2 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

12 April 2014 9,296 30 View

What is the best way to preserve M.tuberculosis?

I have tried to preserve M. tuberculosis in glycerol and kept at -20C. On subculturing on LJ the recovery has not been good. Also strains die on repeated subculturing on LJ. Can anybody give a...

20 November 2013 5,049 5 View