Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Using SILVACO to design tandem organic solar cells?

Hi Can anyone please suggest me how can I study the parameters of solar cells made of graphene oxide and any other organic material composite using SILVACO. I would also like to know how I can add...

15 February 2021 5,795 3 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

About XRD details of pristine and doped RGO?

Hi Can anyone please help me know the XRD details of reduced graphene oxide, Nitrogen doped reduced graphene oxide, Sulphur doped reduced graphene oxide, Nitrogen and Sulphur co-doped reduced...

08 February 2021 3,033 3 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View

Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Why is there Significant Kruskall Wallace significant man Whitney u insignificant But Dunn post hoc?

08 May 2020 7,979 4 View

Why Low bead count or no bead count while running 13 Plex on Luminex Magpix instrument?

Hello Everyone! Yesterday i was analyzing 13 Plex by using Luminex Magpix Instrument. In the morning the Cal & Ver was failed, I sonicate the probe, perform enhance statrup routine than Cal...

05 June 2018 3,600 2 View

How do you compute for normalized standard deviation (NSD) from Elovich kinetics model parameters?

the NSD requires q(cal) (mg/g) but Elovich model only has alpha (mg/g.min) and beta (g/mg)

05 May 2018 8,093 4 View

How do you compute for normalized standard deviation (NSD) from Elovich kinetics model parameters?

the NSD requires q(cal) (mg/g) but Elovich model only has alpha (mg/g.min) and beta (g/mg)

04 May 2018 1,487 4 View

I'm trying to quantify an active on HPLC, but I'm getting a lower result than I should. What should I check?

I have run a 5 cal curve. 21.5, 43, 86,129, and 172 mg/100 ml of active in methanol. My sample should read 29-30 mg/100 ml. I get about 3 mg/100 ml every time. What's wrong? I'm afraid I'm...

03 April 2018 7,542 11 View

Is Cal 520 AM labling neuron or glial cell?

any one ever worked with this dye before? any suggestion is helpful. thx

03 April 2018 202 2 View

Someone help and simplify how to calculate KL (Langmuir) , K1, K2, Qe cal (pseudo-first and pseudo second) Given the slope and intercept?

Simply describe how to use the slope and intercept from the plotted graph to compute for those parameters.

02 March 2018 106 1 View

Research partners for a PRIMA proposal?

We, in Algeria, are actively searching partners from Europeans countries in order to submit a proposal in animal or food field: Topics: 2.2 Preventing emergence of animal and plant pests and...

02 March 2018 7,328 9 View

Where cal I find free big data sets in education field?

I want to develop a Big Data model for implementing BI in educational institutes but I don't have enough data for it.

31 December 2017 4,686 0 View

Difference between large calories and small calories?

As cal is defined as the approximate amount of energy needed to raise the temperature of one gram of water by one degree Celsius at a pressure of one atmosphere. It would be nice if someone can...

09 October 2017 208 2 View