Изолирована от крови. Рост при 25 ° С на среде Сабуро. На 14 день рост составил 5,5 см в диаметре. Сравнение данных из микроскопической морфологии культуры и тканевой формы гриба в крови пациента позволяет выявить диморфизм грибов из рода Paecilomyces, о которых я расскажу после идентификации.
Dear Alima Akhunova,
Paecilomyces spp. are particularly widespread soil saprophytes. Five species Paecilomyces varioti, P. lilacinus, P. marquarti, P. viridis , and P. japonicus have been reported from human infection. P. varioti was identified in numerous blood cultures.
For identification of Paecilomyces spp. I need a macrophoto (the growth on Petri dishes) and microphoto (a microscope preparate slide) from a cultivation on MEA medium at 25 ° C, 14 days.
Best regards
Vladimir
Dear Vladimir! Thank you for your attention to my request.
The information you have requested, presented in the attachment.
Sincerely Alima Akhunova.
Dear Alima Akhunova,
for Paecilomyces spp. identification you need the macrophoto (the growth on Petri dishes) and the microphoto (microscope preparate slide) from the cultivation on MEA (Malt Extract Agar) at 25 ° C, 14 days. The comparison of Paecilomyces lilacinus, P. marquarti and P. varioti can you see in attachment (the information from dr. Alena Kubatova, Charles University in Prague, Faculty of Science, Department of Botany, https://www.natur.cuni.cz/biology/botany/kubatova?set_language=en)
My best regards
Vladimir
Dear Vladimir! Thank you. I'll take your advice.
Sincerely Alima Akhunova.
Dear Alima Akhunova
For Paecilomyces spp. identification, you need the cultural, macro and the microscopic characteristics from the cultivation on MEA (Malt Extract Agar) at 25 ° C, 14 days. However, molecular characters have played an important role in the detection and identification of Paecilomyces spp. So to confirm morphological identification you have to use of molecular methods based on ITS and TEF genes.
Best regards: Hamid
Dear Alima
The colony photographs & micro photographs of mycelia and spores are not sufficient for the identification. Some more morphological and structural details of sporulation bodies will help in identification .
Dear Ghanshyam Tandon! Thank you. Sincerely Alima Akhunova.
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