Hello Moumita,

The input file is fairly easy:

_ first line: marker names separated by space

_ second line (optional): phase information, ...

_ all next lines are your individual (name, population, genotype at the markers)

The best way to prepare your file (in my experience) from a crude genotype file is to use the MS-toolkit in excel (Park 2001), convert the file to a Fstat format and copy paste the individual genotypes to a new notepad. Write the name of your markers on the first line, and additional informations if needed in the next lines. Then, you just have to indicate which information is present/absent when you start your project in Structure.

Please read the documentation of the software for more information ( http://pritchardlab.stanford.edu/structure_software/release_versions/v2.3.4/structure_doc.pdf ).

I also attached an animated presentation I've made for teaching how to use the software to our undergraduate students.

Hope this is helpful! Let me know if you have more questions.

Cheers,

Alex

There are several possible file formats depending upon the information that you choose to include and the way your genotype matrix is organized. The manual does a good job of describing these, and other important details about the program. I highly recommend reading it. 

can i get the input file format for binary data

My data are arranged basing on GenomeStudio whereby the 1st row is number of samples and 1st column consists SNPs. Then individuals have several information for Gtype, Theta, Score and R. Any one who can help on how to go through!

Hiiii Mr Suchandranath Babu Munnam

Mr Alexis Beaurepaire already explain it very well,

herewith i send an example of input data and how to input (in doc).

i hope it can help you.

Can anyone help me how to imput file DNA seq data in Structure format...

And Popart input data also for DNA seq..including 'trait lebel' input data.

Plz help..

Column 1. Genotypes Labell

Column 2 Putative population number

Column 3 and onward Trait data

Dear Sir thank you for your suggestion..Sir its my request if i give you a simplified raw data in Fasta format and tell you the population size and number of seq. contain in each population ..can u plz help me to prepare a popart nexus file format...

May i hv your email id.

Mine is : [email protected]

I can help some for setting structure analysis

For structure software the input file format is make the file with binary data in excel save it in tab (delimited) form in excel ...it will open in notepad ..directly load your file by opening new project in structure software....

Yes I can help u. See this format, then save as tab delimited. You can see my paper

Article Diversity and population structure of red rice germplasm in Bangladesh

@panna Ali sir I have fasta files of sequences how to load these files into structure software....

Hi

I am converting .ped file to structure format It is duplicating the no of samples. I had 96 samples but in structure file it is showing 192 samples. Same is with no of markers. I had 30,000 in .ped file but in structure file it is showing 60, 000..

Can anyone please help??

Yes. I can help you. Please use the attached format. Then try.

Thank you. Will try with the given format..

You can use nexus file for Popart. The fasta file can be converted into nexus file in DNAsp after haplotype analysis.

Hi Everyone,

I am doing genotyping and Population genetic study in Tea with polymorphic SSR markers. I used 10 polymorphic SSRs. As heterozygous nature, tea is giving multiple bands for a SSR marker. Please anyone help me, how to input data into Excel file which can be used to perform population study using STRUCTURE software.

Regards

Jeevan

Hi

Thanks. See my previous attached data format.

Thank you very much Dr. Ali

I am continously getting an error window saying there is error in second line. I tried all possible ways to correct it but failed. Please suggest what could be done..